Planta Med 2005; 71(9): 819-824
DOI: 10.1055/s-2005-871249
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Immunomodulatory Activity and Influence on Mitotic Divisions of N-Benzoylphenylisoserinates of Lactarius Sesquiterpenoid Alcohols in vitro

Ewa Krawczyk1 , Mirosław Łuczak1 , Monika Kniotek2 , Anna Majewska1 , Dariusz Kawecki1 , Maria Nowaczyk2
  • 1Chair and Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland
  • 2Department of Clinical Immunology, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland
Further Information

Publication History

Received: November 20, 2004

Accepted: April 15, 2005

Publication Date:
29 July 2005 (online)

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Abstract

Six N-benzoylphenylisoserinates of Lactarius sesquiterpenoid alcohols, which previously showed antiviral activities, were tested for their biological properties. Their influence on the mitotic division of the cells and on selected immunological parameters, e. g., T and B lymphocyte proliferation and synthesis of the cytokines: interleukin 2 (IL-2), tumour necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) was assessed in vitro. All of the tested compounds significantly decreased the number of cell divisions. It appears that their influence on cellular divisions may be associated with anti-HSV activity. Moreover, one compound - isolactarorufin 8-epi-[N-benzoyl-(2′R,3′S)-3′-phenylisoserinate] significantly inhibited T lymphocyte proliferation and the synthesis of all tested cytokines.

Abbreviations

DMSO:dimethyl sulphoxide

FCS:foetal calf serum

HEPES:4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid

MEM:minimum essential medium

PBMC:peripheral blood mononuclear cells

PBS:phosphate buffered saline

PHA:phytohaemagglutinin

PMA:phorbol myristate acetate

SAC:Staphylococcus aureus Cowan (strain)

References

Ewa Krawczyk

Chair and Department of Medical Microbiology

Medical University of Warsaw

5 Chalubinski St.

02-004 Warsaw

Poland

Phone: +48-22-622-0028

Fax: +48-22-628-2739

Email: ewakrawczyk@gazeta.pl